ABSTRACT
In order to advance the treatment of Alzheimer's disease with active ingredients of Chinese herbal medicines and the research on these ingredients and their effective targets in treating the disease, the relative representative literatures published in recent years were reviewed and summarized in this paper.
Subject(s)
Humans , Alkaloids , Alzheimer Disease , Drug Therapy , Drugs, Chinese Herbal , Chemistry , Therapeutic Uses , Ginkgo biloba , Chemistry , Ginsenosides , Therapeutic Uses , Phytotherapy , Methods , Salvia miltiorrhiza , Chemistry , Sesquiterpenes , Therapeutic UsesABSTRACT
<p><b>OBJECTIVE</b>To establish a method for fingerprinting of Fuzhisan (FZS, a traditional Chinese medicinal preparation) using high-performance liquid chromatography with ultraviolet and evaporative light scattering detector (HPLC-UV/ELSD) to allow simultaneous determination of 5 major constituents in the preparation.</p><p><b>METHODS</b>HPLC-UV/ELSD analysis was performed on water AlltechC18 column (5 microm, 4.6 mm x 250 mm) with a mixture of acetonitrile (A) and 0.1% acetice acid water (B) as the mobile phase. The solvent A gradient for elution was 0, 12%; 25, 20%; 30, 20%; 75, 30%; 105, 40%; 120, 80%; 130, 12%, with the flow rate of 1.0 ml/min; and the column temperature at 30 degrees . The detective wavelength was 335 nm, drift tube temperature was 80 degrees , pressure of nebulizer gas was 25 psi. The similarities between the HPLC-UV/ELSD fingerprints of the 12 extracts were calculated using similarity evaluation software.</p><p><b>RESULTS</b>The fingerprint of FZS was established and the 5 major constituents were identified. The complementarity between the fingerprints of UV and ELSD was analyzed, showing good correlation between 12 batches of FZS.</p><p><b>CONCLUSION</b>The method for fingerprinting can simultaneously characterize the main chemical constituents in FZS and allows stable, effective and comprehensive quality control and evaluation of FZS for a single sample.</p>
Subject(s)
Chromatography, High Pressure Liquid , Methods , Drugs, Chinese Herbal , Chemistry , Reference Standards , Light , Quality Control , Scattering, Radiation , Ultraviolet RaysABSTRACT
Objective To investigate the therapeutic action of Taurine in patients with cerebral infarction(CI)induced by hyperhomocysteinemia(Hhcy).Methods The 100 CI patients induced by Hhcy were randomly divided to two therapy groups.One group was administered Taurine,the other group was administered Folacin combined with Vitamine B12 for 8 weeks.Before and after treating,the serum levels of homocysteic acid(Hcy),Folacin and Vitamine B12 were detected,NIHSS and BI were evaluated.Results The serum levels of Hcy were significantly decreased after administering in both two groups(all P